Journal: Virulence

Article Title: The PirB toxin protein from Vibrio parahaemolyticus induces apoptosis in hemocytes of Penaeus vannamei

doi: 10.1080/21505594.2021.1872171

Figure Lengend Snippet: Summary annotated proteins identified to interact with PirB

Article Snippet: The collected supernatant was analyzed using SDS-PAGE and Western blot, as described in subsection 2.2, with mouse anti-histone H3 antibody (1:1000, Transgen BioTech, Beijing, China), and rabbit anti-phospho histone H3 (Ser10) antibody (1:1000, Cell Signaling Technology, MA, USA).

Techniques:

Journal: Virulence

Article Title: The PirB toxin protein from Vibrio parahaemolyticus induces apoptosis in hemocytes of Penaeus vannamei

doi: 10.1080/21505594.2021.1872171

Figure Lengend Snippet: The interaction between PirB toxin protein and histones. (a) Purification of nucleosome proteins by gel filtration chromatography. (b) SDS-PAGE, Native-PAGE and Western blot analysis of purified nucleosomes. (c) Native-PAGE and Western blot analysis of the interaction between His-rPirB and nucleosome proteins. Purified nucleosomes treated with different concentrations (0.2, 0.1, and 0.05 µM) of recombinant His-rPirA or His-rPirB proteins and analyzed by Native-PAGE and Western blot using anti-histone H3 antibody

Article Snippet: The collected supernatant was analyzed using SDS-PAGE and Western blot, as described in subsection 2.2, with mouse anti-histone H3 antibody (1:1000, Transgen BioTech, Beijing, China), and rabbit anti-phospho histone H3 (Ser10) antibody (1:1000, Cell Signaling Technology, MA, USA).

Techniques: Purification, Chromatography, SDS Page, Clear Native PAGE, Western Blot, Recombinant

Journal: Virulence

Article Title: The PirB toxin protein from Vibrio parahaemolyticus induces apoptosis in hemocytes of Penaeus vannamei

doi: 10.1080/21505594.2021.1872171

Figure Lengend Snippet: The PirB toxin protein induces dephosphorylation of histone H3 on Serine 10. Primary hemocytes were treated with His-rPirB or His-rPirA for 2 h, followed by Western blot analysis to detect total tubulin, histone H3, and phosphorylation levels of histone H3 on Serine 10

Article Snippet: The collected supernatant was analyzed using SDS-PAGE and Western blot, as described in subsection 2.2, with mouse anti-histone H3 antibody (1:1000, Transgen BioTech, Beijing, China), and rabbit anti-phospho histone H3 (Ser10) antibody (1:1000, Cell Signaling Technology, MA, USA).

Techniques: De-Phosphorylation Assay, Western Blot

Journal: Virulence

Article Title: The PirB toxin protein from Vibrio parahaemolyticus induces apoptosis in hemocytes of Penaeus vannamei

doi: 10.1080/21505594.2021.1872171

Figure Lengend Snippet: A proposed model shows that PirB induces apoptosis in shrimp hemocytes. When primary hemocytes of Penaeus vannamei are treated with the Pir proteins from Vibrio parahaemolyticus, the PirB protein enters the cytoplasm and nuclei. Once in the nucleus, the PirB protein interacts with histone proteins to dephosphorylate histone H3 and therefore induce apoptosis in hemocytes

Article Snippet: The collected supernatant was analyzed using SDS-PAGE and Western blot, as described in subsection 2.2, with mouse anti-histone H3 antibody (1:1000, Transgen BioTech, Beijing, China), and rabbit anti-phospho histone H3 (Ser10) antibody (1:1000, Cell Signaling Technology, MA, USA).

Techniques: